loading page

Effect of captopril on paraplegia caused by Spinal Cord Ischemia-Reperfusion Injury in rats
  • Hossein Kazemi,
  • Bahareh Hafezi,
  • Amir Moghadam Jafari
Hossein Kazemi
Ferdowsi University of Mashhad Faculty of Veterinary Medicine

Corresponding Author:[email protected]

Author Profile
Bahareh Hafezi
Ferdowsi University of Mashhad Faculty of Veterinary Medicine
Author Profile
Amir Moghadam Jafari
Ferdowsi University of Mashhad Faculty of Veterinary Medicine
Author Profile

Abstract

Objective: To determine whether captopril could prevent spinal cord damage after induction of ischemia-reperfusion injury in rats. Materials and Methods: We randomly divided 24 adult male Wistar rats, weighing 230 - 350 g, into 4 groups (n= 6 in each group): Spinal cord ischemia-reperfusion with captopril, spinal cord ischemia-reperfusion, sham operated with captopril, and SHAM. 24 and 1.5 hours before ischemia induction, captopril was administered intragastrically (100 mg/kg) to the SHAM+Cap and the SCI-R+Cap groups. Abdominal aortic clamping was performed in the SCI-R and SCI-R+Cap groups for 40 minutes. Hindlimb motor function was evaluated using the Tarlov Scale at 4, 6, 12, 24, 48, and 60 hours after SCI. Biomarkers of oxidative stress were measured to evaluate biological responses to therapeutic interventions (MDA, FRAP and PAB assays). Results: Throughout the study period, the SCI-R group had significantly lower motor function scores than the other groups (P < 0.05). The MDA and PAB levels were higher in the SCI-R group than in the SHAM group (P < 0.05). The FRAP value was lower in the SCI-R group than that in the SHAM group (P < 0.05). The SCI-R+Cap had higher motor function scores than the SCI-R group at all time points (P < 0.05). There were no notable differences in MDA concentration, FRAP and PAB values between the SCI-R+Cap and the SCI-R groups (P > 0.05). Conclusion: Captopril may act as a protective agent against spinal cord ischemia-reperfusion injury in rats based on hind limb motor function assessment.