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Fabrication and Integration of a Low-cost 3D Printing-based Glucose Biosensor for Bioprinted Liver-on-a-chip
  • Jaehee Lee,
  • Somnath Maji,
  • Hyungseok Lee
Jaehee Lee
Kangwon National University
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Somnath Maji
Kangwon National University
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Hyungseok Lee
Kangwon National University

Corresponding Author:[email protected]

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In the last two decades, there have been significant advancements in the development of more physiologically relevant organ-on-a-chip (OOC) systems that can mimic the tissue microenvironment. Despite the advantages of these microphysiological systems, such as portability, the ability to mimic physiological flow conditions, and the reduction of reagents required for preparation and detection, they lack real-time detection of analytes with high accuracy. To address this, biosensor technologies have been integrated with OOC systems to enable simultaneous analysis of different analytes in a single device. However, integrating biosensors with OOC systems is challenging due to the competing demands for low-cost, simple fabrication processes, and speed. This study presents the fabrication of a glucose sensing device integrated with a liver-on-a-chip (LOC) platform. The conductive PLA-based three-electrode system was printed using FDM 3D printing technology to simplify the fabrication process. The sensitivity of the glucose biosensing device was enhanced by adding multi-walled carbon nanotubes on the electrodes. The biosensing integration study using a perfusion-based LOC showed the stability, biocompatibility, and sensitivity of the glucose sensing devices. Furthermore, drug toxicity studies on the LOC platform demonstrated the device’s ability to detect a broad range of glucose concentrations and its enhanced sensitivity.
06 Apr 2023Submitted to Biotechnology Journal
07 Apr 2023Submission Checks Completed
07 Apr 2023Assigned to Editor
17 Apr 2023Reviewer(s) Assigned
13 May 2023Review(s) Completed, Editorial Evaluation Pending
15 May 2023Editorial Decision: Revise Major
10 Aug 20231st Revision Received
14 Aug 2023Submission Checks Completed
14 Aug 2023Assigned to Editor
14 Aug 2023Reviewer(s) Assigned
22 Aug 2023Review(s) Completed, Editorial Evaluation Pending
23 Aug 2023Editorial Decision: Accept