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Native Capillary Electrophoresis-Mass Spectrometry for Protein analysis: Current Progress and Perspectives
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  • Ann-Katrin Schwenzer,
  • Lena Kruse,
  • Kevin Jooß,
  • Christian Neusüß
Ann-Katrin Schwenzer
Aalen University of Applied Sciences
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Lena Kruse
Aalen University of Applied Sciences
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Kevin Jooß
Northwestern University

Corresponding Author:[email protected]

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Christian Neusüß
Aalen University of Applied Sciences
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Native mass spectrometry is a rapidly emerging technique for fast and sensitive structural analysis of protein constructs, maintaining the protein higher order structure. The coupling with electromigrative separation techniques under native conditions enables the characterization of proteoforms and highly complex protein mixtures. In this review, we present an overview of current native CE-MS technology. First, the status of native separation conditions is described for capillary zone electrophoresis (CZE), affinity capillary electrophoresis (ACE), and capillary isoelectric focusing (CIEF), as well as their chip-based formats, including essential parameters such as electrolyte composition and capillary coatings. Further, conditions required for native ESI-MS of (large) protein constructs, including instrumental parameters of QTOF and Orbitrap systems, as well as requirements for native CE-MS interfacing are presented. On this basis, methods and applications of the different modes of native CE-MS are summarized and discussed in the context of biological, medical, and biopharmaceutical questions. Finally, key achievements are highlighted and concluded, while remaining challenges are pointed out.
10 Mar 2023Submitted to PROTEOMICS
13 Mar 2023Assigned to Editor
13 Mar 2023Submission Checks Completed
13 Mar 2023Review(s) Completed, Editorial Evaluation Pending
14 Mar 2023Reviewer(s) Assigned
14 Apr 2023Editorial Decision: Revise Minor
16 May 2023Review(s) Completed, Editorial Evaluation Pending
16 May 20231st Revision Received
23 May 2023Editorial Decision: Accept