Three-dimensional (3D) printed bioactive scaffolds have been widely used
in the field of bone tissue engineering. However, its visualization in
vivo and bacterial inflammation are intractable issues during the
surgery and treatment. Herein, we firstly synthesized an
aggregation-induced emission-active luminogen (AIEgen), named as 4BC,
with efficient reactive oxygen species (ROS) generation. Then, a series
of 3D bioactive scaffolds loading 4BC were fabricated by precipitation
adsorption method, namely 4BC@scaffolds, which showed good in situ
imaging performance for the implanted scaffolds by using simple UV light
irradiation. Among them, 4BC@TMP scaffold composed of trimagnesium
phosphate (TMP) had an excellent bactericidal ability for E. coli and S.
aureus in vitro and resisted bacterial inflammation in vivo through
photodynamic action. H&E and immunofluorescence staining were performed
to further evaluate the inhibitory effect of bacterial inflammation in
vivo. This work verified that AIEgen-based 3D scaffolds are promising
bioactive frameworks for bioimaging and antibacterial application.