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Galantamine alleviates cognitive dysfunction in aged mice by inhibiting endoplasmic reticulum stress
  • Tao Jia,
  • Xiaobo Zhu,
  • Jinliang Teng
Tao Jia
Hebei North University

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Xiaobo Zhu
Hebei North University
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Jinliang Teng
The First Affiliated Hospital of Hebei North University
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Abstract

Objective: The risk of postoperative cognitive dysfunction in elderly patients is high, affecting work and life, and the quality of life is decreased to some extent. However, there is an urgent need to discover a new therapeutic target drug to prevent POCD occurrence. Galantamine, a cholinesterase inhibitor, can reduce apoptosis and relieve POCD by inhibiting endoplasmic reticulum stress. Our study will investigate the mechanism of galantamine on isoflurane induced POCD in aged mice. Methods: The aim of this study was to construct a POCD model of aged mice, test the effects of isoflurane on learning and memory of aged mice by using Y maze and open field equipment, and then analyze the apoptosis rate of hippocampal CA1 neurons by TUNEL method. The mRNA and protein expressions of GRP78, ATF4 and CHOP were determined by RT-qPCR and Western-Blot. Through experimental analysis, this study also obtained the optimal dose of galantamine in the treatment of POCD. Results: Our experimental results showed that after isoflurane anesthesia, the cognitive function of aged mice was significantly impaired, the neuronal cells in the hippocampus were seriously apoptotic, and GRP78, ATF4 and CHOP were upregulated. It is worth noting that our study confirmed that galantamine saved neuronal cell apoptosis in aged mice and drastically down-regulated GRP78, ATF4 and CHOP, which is expected to be a new pharmacological target for the treatment of POCD. Interpretation: Galanthamine is a drug with wide application space in the field of postoperative cognitive impairment research. This study explores pharmacological targets for improving cognitive dysfunction