Background and Objective: Pulse diagnosis is the most characteristic method of diagnosis in traditional Chinese medicine and contains a wealth of pathophysiological information. This study aimed to investigate the acoustic characteristics of the pulses of pregnant women in mid- to late-term pregnancy (pregnant women) and relatively healthy women without pregnancy (non-pregnant women). The pulse of the wrist was divided into three parts: the Cun, Bar, and Cubit, each of which was divided vertically into five layers. The perspective of the acoustic factors in the pulse was analyzed to compare the differences in the acoustic pulse of pregnant and non-pregnant women. Methods: The acoustic characteristics of the pulse were analyzed using wavelet analysis and empirical modal decomposition by combining multi-scale entropy and Shannon entropy, and the differences between the two groups were examined. Receiver operating characteristic (ROC) curves were plotted to judge the sensitivity and accuracy of the acoustic wave characteristics for the preliminary diagnosis of pregnancy in women. Results: The parameters of the third and fourth layers of the Cubit pulse in pregnant women were significantly different to those of non-pregnant women. The multi-scale entropy, Shannon entropy values, energy, and acoustic wave amplitude of the pulses of pregnant women were higher than those of non-pregnant women, which indicates that pregnant women had more complex waveform. The acoustic wave characteristics of pregnant women mainly manifested in the EG and IJ segments of the third layer of the Cubit pulse, and IJ segment of the fourth layer of the Cubit pulse