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Statistical approach for lipase production from thermotolerant Bacillus subtilis TTP-06 isolated from a hot spring
  • Manpreet Kaur,
  • Reena Gupta
Manpreet Kaur
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Reena Gupta
Himachal Pradesh University, Shimla

Corresponding Author:[email protected]

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In present study, response surface methodology was employed for the optimization of production of extracellular lipase from thermotolerant Bacillus subtilis TTP-06 which was isolated from Tattapani hot spring of Himachal Pradesh, India. In Plackett-Burman Design (PBD), interactions among six different factors viz. glucose and peptone concentration; pH, temperature, incubation time and olive oil concentration were studied. The factors which showed a positive effect on the production included glucose and nitrogen concentration; pH and temperature. The optimum values of all these positive factors were determined by using Central Composite Design (CCD). Results of statistical analysis of variance (ANOVA) indicated a p-value of <0.05 which revealed the significance of quadratic model with determination coefficient (R2) value of 0.7846. The optimum concentration of glucose 2.75% (w/v) and peptone 1.65% (w/v) in a medium of pH 7.5 incubated at temperature 55ºC for 24 hrs with 1% (v/v) inoculum gave maximum lipase activity of 8.889 U/ml.