loading page

Generation and cloning of CH2 knockout cats by CRISPR-Cas9 system: Hypoallergenic cats
  • +8
  • Sang Ryeul Lee,
  • Kyung-Lim Lee,
  • Seok-Hwan Song,
  • Myeong-Don Joo,
  • Seo-Hyun Lee,
  • Ji-Su Kang,
  • Seon-Min Kang,
  • Muhammad Idrees,
  • Jae-Wook Kim,
  • Jonghyeok Ko,
  • Il-Keun Kong
Sang Ryeul Lee
Utah State University Department of Animal Dairy and Veterinary Sciences
Author Profile
Kyung-Lim Lee
Gyeongsang National University
Author Profile
Seok-Hwan Song
Gyeongsang National University Institute of Agriculture and Life Science
Author Profile
Myeong-Don Joo
Gyeongsang National University Institute of Agriculture and Life Science
Author Profile
Seo-Hyun Lee
Gyeongsang National University Institute of Agriculture and Life Science
Author Profile
Ji-Su Kang
Gyeongsang National University Institute of Agriculture and Life Science
Author Profile
Seon-Min Kang
Gyeongsang National University Institute of Agriculture and Life Science
Author Profile
Muhammad Idrees
Gyeongsang National University Institute of Agriculture and Life Science
Author Profile
Jae-Wook Kim
Gyeongsang National University
Author Profile
Jonghyeok Ko
Gyeongsang National University
Author Profile
Il-Keun Kong
Gyeongsang National University

Corresponding Author:[email protected]

Author Profile

Abstract

Background: Allergy health problems worldwide are mainly caused by allergens from domestic cats ( Felis catus). Fel d 1 is a major allergen that causes severe allergic reactions in humans, including rhinitis, conjunctivitis, and life-threatening asthma.out cats. Fel d 1 level in CH2 knockout cats was assessed by enzyme-linked immunosorbent assay (ELISA). Cytoplasm injection clone technology (CICT) was used to clone the CH2 knockout cat. Results: We report the first successful generation and cloning of CH2 knockout cats using the CRISPR-Cas9 system and CICT. CH2 knockout cats were confirmed using T7E1 and Sanger sequencing. Cloned CH2 knockout cat was verified by microsatellite analysis. Remarkably, ELISA proved that Fel d 1 level of CH2 knockout cats was extremely low compared with that of wild type domestic cats. Conclusion: CH2 knockout cats we generated showed an extremely low level of Fel d 1 and could be hypoallergenic cats. Our study indicates that creating hypoallergenic cats using the CRISPR-Cas9 system is a significant step forward in the consequence that these cats can safely approach allergic patients.
29 Feb 2024Published in Scientific Reports volume 14 issue 1. https://doi.org/10.1038/s41598-024-55464-0