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CsCuAO1 associated with CsAMADH1 confers drought tolerance by modulating GABA levels in the tea plant
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  • XUJUN ZHU,
  • Yu Cao,
  • Kexin Zhang,
  • Yiwen Chen,
  • Yu Duan,
  • Zhongwei Zou,
  • Qiang Shen,
  • Yuanchun Ma,
  • WANPING FANG
XUJUN ZHU
Nanjing Agricultural University College of Horticulture

Corresponding Author:[email protected]

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Yu Cao
Nanjing Agricultural University College of Horticulture
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Kexin Zhang
Nanjing Agricultural University College of Horticulture
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Yiwen Chen
Nanjing Agricultural University College of Horticulture
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Yu Duan
Nanjing Agricultural University College of Horticulture
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Zhongwei Zou
Wilfrid Laurier University Department of Biology
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Qiang Shen
Guizhou Provincial Academy of Agricultural Sciences
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Yuanchun Ma
Nanjing Agricultural University College of Horticulture
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WANPING FANG
Nanjing Agricultural University College of Horticulture
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Abstract

Our previous study has shown that copper-containing amine oxidase ( CuAO) and aminoaldehyde dehydrogenase ( AMADH) could regulate the accumulation of γ-aminobutyric acid (GABA) in tea plants by participating in polyamine degradation pathway. However, the involvement of these genes in drought tolerance has not been underlined. In this study, CsCuAO1 associated with CsAMADH1 confers drought tolerance, which modulates GABA levels in tea plants, was conducted. The results showed that exogenous GABA spraying effectively alleviated the drought induced physical damage. Overexpression Arabidopsis lines of CsCuAO1 and CsAMADH1 exhibited enhanced resistance to drought, which promoted the synthesis of GABA and putrescine, by alerting reactive oxygen species scavenging capacity and stomatal movement. However, suppression of CsCuAO1 or CsAMADH1 in tea plants exhibited increased sensitivity during drought treatment. Moreover, the co-overexpressed plants increased the accumulation of GABA both in the Agrobacterium-mediated Nicotiana benthamiana transient assay and transgenic Arabidopsis plants. In addition, a GABA transporter, CsGAT1, was identified, whose expression is strongly correlated with GABA accumulation levels in different tissues under drought stress. Taken together, CsCuAO1 and CsAMADH1 were involved in response to drought stress through a dynamic balance between GABA and putrescine. Our data will greatly contribute to the characterization of GABA biological functions in response to environmental stresses in plants.