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An efficient whole-cell catalyst for one-pot D-allulose production from glycerol
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  • Hui Zhang,
  • Anqi Zhao,
  • Lingbo Qu,
  • Wenlong Xiong,
  • Md. Asraful Alam,
  • Jixing Miao,
  • Weigao Wang,
  • Jingliang Xu,
  • Yongkun Lv
Wenlong Xiong
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Md. Asraful Alam
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Jixing Miao
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Weigao Wang
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Jingliang Xu
Zhengzhou University
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Yongkun Lv
Zhengzhou University

Corresponding Author:[email protected]

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D-Allulose has many health-benefiting properties, physiological functions, and sustainable applications in food, pharmaceutical, and nutrition industries. The aldol reaction based route is a very promising alternative to Izumoring strategy in D-allulose production. Remarkable studies have been reported in this field, but still suffer from by-product formation and costly purified enzyme involvement. In the present study, we explored the glycerol assimilation, alditol oxidase, alcohol dehydrogenase, aldolase, and dephosphorylation pathways, and modularly designed, assembled, and optimized the D-allulose synthetic cascade in Escherichia coli envelop. We achieved an efficient whole-cell catalyst that produces only D-allulose from cheap glycerol feedstock, eliminating the involvement of purified enzymes. Detailed process optimization improved the D-allulose titer by 1500.00%. Finally, the production was validated in 3-L scale using a 5-L fermenter, and 5.67 g/L D-allulose was produced with a molar yield of 31.43%. This study provided a facile approach to produce D-allulose from glycerol feedstock.
30 Nov 2022Submitted to Biotechnology Journal
30 Nov 2022Submission Checks Completed
30 Nov 2022Assigned to Editor
01 Dec 2022Reviewer(s) Assigned
26 Dec 2022Review(s) Completed, Editorial Evaluation Pending
11 Jan 2023Editorial Decision: Revise Major
01 Mar 20231st Revision Received
05 Mar 2023Submission Checks Completed
05 Mar 2023Assigned to Editor
07 Mar 2023Reviewer(s) Assigned
13 Apr 2023Review(s) Completed, Editorial Evaluation Pending
14 Apr 2023Editorial Decision: Accept