Introduction: Preeclampsia (PE) is a hypertensive disorder of pregnancy (HDP). DLX5 plays an important role in the migration and differentiation of subglobus pallidus precursor cells. Material and methods: We established a zebrafish line expressing high levels of DLX5 and investigated changes in behavior and development of the nervous system. Results: The ratios of brain volume area to whole body area at 96 hpf zebrafish in the experimental group (gRNA+CasRx) were significantly lower than the WT group and the negative control group (casRx)(P<0.01). Behavioral trajectory distances and movement speeds exhibited by the 6th day of development in zebrafish in the experimental group (gRNA+CasRx) were significantly shorter (P<0.01) and lower (P<0.05) than the negative control group (gRNA+CasRx), respectively. Conclusions: Data suggested that the increased expression levels of DLX5 can inhibit brain volume development and behavioral activities in zebrafish. Maybe the high expression levels of DLX5 in the pathological state of PE can inhibit the development of the nervous system in offspring.