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Enzymatic hydrolysis and detection of 3-Monochloropropane-1,2-diol esters
  • ming fang
ming fang
Wuhan Polytechnic University

Corresponding Author:[email protected]

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There are more and more studies on the detection method of 3-chloro-1,2-propanediol fatty acid esters (3-MCPD esters) at present. By comparing these methods for the determination of 3-MCPD esters. Indirect methods, which determine total amount of 3-MCPD after hydrolysis of the esters, have an advantage over direct methods. The existing indirect methods, however, may yield unreliable results or require long hours of alkaline methanolysis. In contrast, the Indirect enzymatic hydrolysis method has mild conditions and more accurate results. In this study, we developed a reliable and rapid indirect method for determinations of 3-MCPD esters. 3-MCPD esters was enzymatized to 3-MCPD by indirect enzymatic hydrolysis method, and the conditions of enzymatic hydrolysis were optimized, the content of 3-MCPD after enzymatic hydrolysis was detected by gas chromatography-mass spectrometry (GC-MS) and the yield was calculated. Finally, the optimum conditions for enzymatic hydrolysis of 3-MCPD esters were determined. According to the optimal enzymatic hydrolysis condition, the contents of 3-MCPD esters in four food oils were determined. The method is simple and sensitive, and can meet the requirement of 3-MCPD esters detection in general oils.
11 Nov 2022Submitted to Journal of the American Oil Chemists' Society
14 Nov 2022Submission Checks Completed
14 Nov 2022Assigned to Editor
15 Nov 2022Review(s) Completed, Editorial Evaluation Pending
16 Dec 2022Reviewer(s) Assigned
07 Feb 2023Editorial Decision: Revise Major
09 Mar 20231st Revision Received
09 Mar 2023Submission Checks Completed
09 Mar 2023Assigned to Editor
09 Mar 2023Review(s) Completed, Editorial Evaluation Pending
15 Mar 2023Reviewer(s) Assigned
12 Apr 2023Editorial Decision: Accept