There are more and more studies on the detection method of
3-chloro-1,2-propanediol fatty acid esters (3-MCPD esters) at present.
By comparing these methods for the determination of 3-MCPD esters.
Indirect methods, which determine total amount of 3-MCPD after
hydrolysis of the esters, have an advantage over direct methods. The
existing indirect methods, however, may yield unreliable results or
require long hours of alkaline methanolysis. In contrast, the Indirect
enzymatic hydrolysis method has mild conditions and more accurate
results. In this study, we developed a reliable and rapid indirect
method for determinations of 3-MCPD esters. 3-MCPD esters was
enzymatized to 3-MCPD by indirect enzymatic hydrolysis method, and the
conditions of enzymatic hydrolysis were optimized, the content of 3-MCPD
after enzymatic hydrolysis was detected by gas chromatography-mass
spectrometry (GC-MS) and the yield was calculated. Finally, the optimum
conditions for enzymatic hydrolysis of 3-MCPD esters were determined.
According to the optimal enzymatic hydrolysis condition, the contents of
3-MCPD esters in four food oils were determined. The method is simple
and sensitive, and can meet the requirement of 3-MCPD esters detection
in general oils.