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Geobacter sulfurreducens metabolism at different donor/acceptor ratios
  • Hanna Marianne Frühauf-Wyllie,
  • Dirk Holtmann
Hanna Marianne Frühauf-Wyllie
DECHEMA Forschungsinstitut
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Dirk Holtmann
Technische Hochschule Mittelhessen
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Abstract

Geobacter species have great application potential in remediation processes and electrobiotechnology. In all applications, understanding the metabolism will enable target-oriented optimization of the processes. The typical electron donor and carbon source of the Geobacter species is acetate, while fumarate is the usual electron acceptor. Here, we could show that depending on the donor/acceptor ratio in batch cultivation of G. sulfurreducens different product patterns occur. With a donor/acceptor ratio of 1:2.5 malate accumulated as an intermediate product but was metabolized to succinate subsequently. At the end of the cultivation, the ratio of fumarate consumed and succinate produced was approximately 1:1. When fumarate was added in excess, malate accumulated in the fermentation broth without further metabolization. After the addition of acetate to stationary cells, malate concentration decreased immediately and additional succinate was synthesized. Finally, it was shown that also resting cells of G. sulfurreducens could efficiently convert fumarate to malate without an additional electron donor. Overall, it was demonstrated that by altering the donor/acceptor ratio, targeted optimization of the metabolite conversion by G. sulfurreducens can be realized.
22 Jul 2022Submitted to MicrobiologyOpen
25 Jul 2022Submission Checks Completed
25 Jul 2022Assigned to Editor
28 Jul 2022Reviewer(s) Assigned
27 Aug 2022Review(s) Completed, Editorial Evaluation Pending
30 Aug 2022Editorial Decision: Revise Major
02 Sep 20221st Revision Received
05 Sep 2022Submission Checks Completed
05 Sep 2022Assigned to Editor
05 Sep 2022Review(s) Completed, Editorial Evaluation Pending
05 Sep 2022Editorial Decision: Revise Minor
11 Sep 20222nd Revision Received
12 Sep 2022Submission Checks Completed
12 Sep 2022Assigned to Editor
12 Sep 2022Review(s) Completed, Editorial Evaluation Pending
12 Sep 2022Editorial Decision: Accept
Oct 2022Published in MicrobiologyOpen volume 11 issue 5. 10.1002/mbo3.1322