1. Stable nucleic acid storage and preservation in resource limited
settings is often a barrier to widespread pathogen surveillance. 2.
Dried Blood Spot (DBS) filter paper has a long history of preserving
nucleic acid in whole blood, we tested extending this technology to the
room temperature storage of DNA extract. 3. We found that DNA extracted
from whole mammalian blood can be stored at room temperature on DBS
filter paper, then washed with the buffer solution Tris-HCL, and used in
downstream PCR analysis with results comparable to PCR performed on DNA
extracted from whole blood dried on DBS filter paper which is the gold
standard. 4. The success of this method means DNA can be stored at room
temperature making sharing genomic samples easier by eliminating the
need for cold chain.