In order to obtain the dominant immunogen of the protein encoded by
p113/p126 family members of Mycoplasma ovipneumoniae. In this
study, p113/p126 family recombinant proteins were prepared. We evaluated
the antibody level induced by the recombinant protein of each member in
the family and its binding ability with the whole bacterial protein. The
binding ability of recombinant protein to whole bacterial protein was
also evaluated. p113/p126 family members were rich in antigen epitopes.
The recombinant proteins (rP128, rP126, and rP113) have the strongest
binding ability to the serum of infected animals and belonged to the
dominant immunogen. Mice immunized with recombinant protein could
produce specific antibodies. The antibody produced by immunizing mice
with recombinant proteins (rP113, rP109, and rP130) can effectively bind
to bacterial protein. In summary, these results provided a basis for
further research on the development of subunit vaccine and the
establishment of serological diagnosis methods.