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Acquisition of hypoxia inducibility by oxygen sensing N-terminal cysteine oxidase in spermatophytes
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  • Daan Weits,
  • Lina Zhou,
  • Laura Dalle Carbonare,
  • Sergio Iacopino,
  • Luca Piccinini,
  • Vinay Shukla,
  • Liem Bui,
  • Giacomo Novi,
  • Joost van Dongen,
  • BEATRICE GIUNTOLI,
  • Francesco Licausi
Daan Weits
Scuola Superiore Sant'Anna
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Lina Zhou
Lanzhou University
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Laura Dalle Carbonare
Scuola Superiore Sant'Anna
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Sergio Iacopino
University of Oxford
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Luca Piccinini
Scuola Superiore Sant'Anna
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Vinay Shukla
University of Geneva
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Liem Bui
Cuu Long Delta Rice Research Institute
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Giacomo Novi
Scuola Superiore Sant'Anna
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Joost van Dongen
RWTH Aachen University
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BEATRICE GIUNTOLI
University of Pisa
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Francesco Licausi
University of Oxford
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Abstract

N-terminal cysteine oxidases (NCOs) use molecular oxygen to oxidize the amino-terminal cysteine of specific proteins, thereby initiating the proteolytic N-degron pathway. To expand the characterization of the plant family of NCOs (PCOs), we performed a phylogenetic analysis across different taxa in terms of sequence similarity and transcriptional regulation. Based on this survey, we propose a distinction of PCOs into two main groups. A-type PCOs are conserved across all plant species and are generally unaffected at the mRNA level by oxygen availability. Instead, B-type PCOs differentiated in spermatophytes to acquire transcriptional regulation in response to hypoxia. The inactivation of two A-type PCOs in Arabidopsis thaliana, PCO4 and PCO5, is sufficient to activate the anaerobic response in young seedlings, whereas the additional removal of B-type PCOs leads to a stronger induction of anaerobic genes and impairs plant growth and development. Our results show that both PCO types are required to regulate the anaerobic response in angiosperms. Therefore, while it is possible to distinguish two clades within the PCO family, we conclude that they all contribute to restrain the anaerobic transcriptional program in normoxic conditions and together generate a molecular switch to toggle the hypoxic response.
15 Jun 2022Submitted to Plant, Cell & Environment
18 Jun 2022Assigned to Editor
18 Jun 2022Submission Checks Completed
20 Jun 2022Reviewer(s) Assigned