Abstract: Objective Patients’ demographics, perioperative data and reasons of using MDT pattern were all analyzed to report preliminary application experiences of an MDT pattern using in the robotic-assisted gynaecological surgery at a single institution. Design Retrospective and descriptive study. Setting Tertiary referral center with one surgical team. Population All the 39 patients who have undergone robotic-assisted MDT gynaecological surgeries from 2018 to 2021 in Chinese PLA General Hospital. Methods Various types of robotic-assisted MDT gynaecological surgeries were carried out by the Da Vinci SI Surgical System. Patients’ demographics, perioperative data and reasons of applying MDT surgeries were described. Main Outcome Measures Patients’ demographics, perioperative data and reasons of applying MDT surgeries. Results Most patients undergone robotic-assisted MDT gynaecological surgeries have at least one abdominal surgery before. The operation cost and hospital expense were higher than common laparoscopy. Hospital stays ranged from 7 to 42 days. General surgery department and urological surgery department were the top two familiar departments involved in robotic-assisted MDT gynaecological surgeries. Only 4 cases appeared intraoperative complications in these surgeries. However, postoperative infection needed to be closely monitored. Conclusions It was indicated that robotic-assisted MDT gynaecological surgeries applied in severe diseases could provide both safety and precision, and the pattern was proved to demonstrate the concept of patient-centered and holistic treatment. Simultaneously, the MDT pattern could cultivate teamwork in various departments in the hospital, thus it was beneficial to both patients and hospitals. Keywords Multi-Disciplinary Teamwork; Robotic-Assisted Surgery; Minimally Invasive Surgery; Gynaecology