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A transcriptional complex of FtMYB102 and FtbHLH4 coordinately regulates the accumulation of rutin in Fagopyrum tataricum
  • +10
  • Dong Zhang,
  • Yaolei Mi,
  • Yu Li,
  • Guangtao Qian,
  • Lucas Vanhaelewyn,
  • Xiangxiao Meng,
  • Tingxia Liu,
  • Wei Yang,
  • Yuhua Shi,
  • Pengda Ma,
  • * Atia-tul-Wahab,
  • Shilin Chen,
  • Wei Sun
Dong Zhang
South China Agricultural University College of Agriculture

Corresponding Author:[email protected]

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Yaolei Mi
South China Agricultural University College of Agriculture
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Yu Li
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Guangtao Qian
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Lucas Vanhaelewyn
Deroose Plants NV Weststraat 129 A 9940 Sleidinge Belgium
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Xiangxiao Meng
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Tingxia Liu
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Wei Yang
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Yuhua Shi
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Pengda Ma
Northwest Agriculture and Forestry University
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* Atia-tul-Wahab
University of Karachi International Center for Chemical and Biological Sciences
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Shilin Chen
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Wei Sun
China Academy of Chinese Medical Sciences Institute of Chinese Materia Medica
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Abstract

Tartary buckwheat ( Fagopyrum tataricum) is rich in flavonoids, which not only play an important role in plant-environment interaction, but are also beneficial to human health. Rutin is a therapeutic flavonol which is massively accumulated in Tartary buckwheat. It has been demonstrated that transcription factors (TFs) control rutin biosynthesis. However, the transcriptional regulatory network of rutin is not fully clear. In this study, through transcriptome and target metabolomics profiling analysis together with homolog prediction, we identified and validated the role of FtMYB102 and FtbHLH4 TFs at the different developmental stages of Tartary buckwheat. The elevated accumulation of rutin in the sprout appears to be closely associated with the expression of FtMYB102 and FtHLH4. Yeast two-hybrid, transient luciferase activity and co-immunoprecipitation analysis demonstrated that FtMYB102 and FtbHLH4 can interact and form a transcriptional complex. Moreover, yeast one-hybrid showed that both FtMYB102 and FtbHLH4 directly bind to the promoter of chalcone isomerase ( CHI), and they can coordinately induce CHI expression as shown by transient luciferase activity assay. Finally, we transferred the FtMYB102 and FtbHLH4 into the hairy roots of Tartary buckwheat and found that they both can promote the accumulation of rutin. Our results indicate that FtMYB102 and FtbHLH4 can form a transcriptional complex by inducing CHI expression to coordinately promote the accumulation of rutin.