Aim Sequencing of the mitogenome of Hygrobates turcicus Pešić, Esen & Dabert, 2017 to expand knowledge of the polymorphism and cryptic or pseudocryptic diversity within Hydrachnidia. Location Bulgaria, Vidima River near Debnewo, 42°56’41.4“N, 24°48’44.6”E, depth 0.4 m, stones on the bottom, water flow 0.71 m/s, temperature 10oC, pH 8.53, oxygen 110 %, conductivity 279 µS/cm, hardness 121 CaO mg/l; 11 males, 27 females, 2 deutonymphs 12.x.2019 leg. Zawal, Michoński & Bańkowska; one male and one female dissected and slides mounted. Methods DNA extraction, sequencing, assembly and annotation, comparison with other populations of H. turcicus, multigene phylogeny Results The mitogenome is 15.006 bp long and encodes for 13 proteins, 2 rRNAs, and 22 tRNAs. The genome is colinear with those of H. longiporus and H. taniguchii, the difference of size originating from a non-coding region located between protein-coding genes ND4L and ND3. Five genes were found to have alternative start-codon, and four display premature termination. The multigene phylogeny obtained using all mitochondrial protein-coding genes unambiguously associates H. turcicus with the cluster formed by H. longiporus and H. taniguchii. Keywords: complete mitogenome, freshwater biodiversity, integrative taxonomy, Trombidiformes, water mites.