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Plug-and-display photo-switchable systems on plant virus nanoparticles
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  • Louisa Kauth,
  • Eva Miriam Buhl,
  • Julian Luka,
  • Karolin Richter,
  • Ulrich Commandeur,
  • Christina Dickmeis
Louisa Kauth
Rheinisch Westfalische Technische Hochschule Aachen Institut fur Biologie VII Molekulare Biotechnologie

Corresponding Author:[email protected]

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Eva Miriam Buhl
Universitatsklinikum Aachen
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Julian Luka
Rheinisch Westfalische Technische Hochschule Aachen Institut fur Biologie VII Molekulare Biotechnologie
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Karolin Richter
Rheinisch Westfalische Technische Hochschule Aachen Institut fur Biologie VII Molekulare Biotechnologie
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Ulrich Commandeur
Rheinisch Westfalische Technische Hochschule Aachen Institut fur Biologie VII Molekulare Biotechnologie
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Christina Dickmeis
Rheinisch Westfalische Technische Hochschule Aachen Institut fur Biologie VII Molekulare Biotechnologie
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Abstract

Light can be used to regulate protein interactions with a high degree of spatial and temporal precision. Photo-switchable systems therefore allow the development of controllable protein complexes that can influence various cellular and molecular processes. Here we describe a plant virus-based nanoparticle shuttle for the distribution of proteins that can be released when exposed to light. Potato virus X (PVX) is often used as a presentation system for heterologous proteins and epitopes, and has ideal properties for biomedical applications such as good tissue penetration and the ability to form hydrogels that present signaling molecules and promote cell adhesion. We describe three different systems attached to the surface of PVX particles: LOVTRAP, BphP1/QPAS1 and Dronpa145N. We demonstrated the functionality of all three photo-switchable protein complexes in vitro and the successful loading and unloading of PVX particles. The new systems provide the basis for promising applications in the biomedical and biomaterial sciences.
21 Oct 2022Published in BioTech volume 11 issue 4 on pages 49. 10.3390/biotech11040049