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Comparative transcriptome analysis of antennae from male crayfish Procambarus clarkii during the mating period and non-mating period
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  • Zihao Zhou,
  • Lili Mo,
  • Wenlong Zeng,
  • Dinghong Li,
  • Hongying Wu,
  • ZHENGJUN WU,
  • Jinlong Huang
Zihao Zhou
Guangxi Normal University

Corresponding Author:[email protected]

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Lili Mo
Guangxi Normal University
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Wenlong Zeng
Guangxi Normal University
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Dinghong Li
Guangxi Normal University
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Hongying Wu
Guangxi Normal University
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ZHENGJUN WU
Guangxi Normal University
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Jinlong Huang
Guangxi Normal University
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Abstract

The red swamp crayfish Procambarus clarkii is one of the global invasive species and has caused huge damage to aquaculture, biodiversity and ecology in the world. Antennal-expressed receptors are important for P. clarkii to detect olfactory cues for mate attraction, et al.. However, few olfactory and chemosensory-related genes were reported in P. clarkii until now. In the present study, we used RNA sequencing to investigate the olfactory and chemosensory-related genes of the antenna of P. clarkii during the non-mating and mating period. A total of 59218 unigenes with an average length of 1056.41 bp, and 4889 differentially expressed unigenes (DEGs) with 2128 up-regulated and 2761 down-regulated unigenes were obtained. Of which, 13 up-regulated and 9 down-regulated DEGs were identified to associated with olfaction and chemical reception, including 4 IRs or iGluRs, 8 G-protein coupled receptor, 5 transient receptor potential channels (TRP channels), 1 sodium-calcium exchanger, 1 olfactory receptor, 1 isomerase and 2 chemosensory proteins (CSPs). CSPs were preliminarily classified into pheromone receptors in male red swamp crayfish. The results of quantitative real-time reverse transcription PCR (RT-qPCR) showed that the expression trends of 8 selected unigenes were consistent, and this result also validated the RNA-Seq data. Our results provide more comprehensive data of olfactory and chemical communication mechanism after crayfish entering the mating period.