Mixed land use provides an important means of promoting the intensive and efficient use of land resources and stimulating endogenous development power in rural areas. This paper selects Pingba Village in Chongqing as the research area, and subdivid the land use types in the rural settlements according to fieldwork results and the principle of dominant function. Moreover, a land mixed use measurement system for rural settlements was constructed to analyze their levels of mixed land use. Furthermore, the influences of the natural environment, social, economic and other factors on mixed land use were comprehensively explored. The results show that 1) the mixed land use of rural settlements in the study area was at a medium level and showed significant spatial variability. 2) The difference in the natural environment determines the level of mixed land use and the basic pattern of its spatial differentiation. Social and economic factors, such as resident population and average household income, are key impact factors. Rural tourism resources, homestead agglomeration policies have important impacts on the level of mixed land use. In conclusion, the research suggests that mixed land use is an important way to boost rural revitalization. In the future, village planning could introduce the concept of mixed land use to improve the efficiency of land use, optimize the land use structure according to local conditions. In addition, it is necessary to scientifically and rationally guide rural settlements to agglomerate appropriately to improve the utilization efficiency of land resources and public service resources.