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Novel method for measuring pulmonary artery pressure by high-frequency ultrasound-guided transthoracic puncture in rats
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  • Xiaofeng Zhang,
  • Chunting Liang,
  • Jingtao Li,
  • Decai Zeng,
  • Yanfen Zhong,
  • Tongtong Huang,
  • Yingying Mo,
  • Huaqing Rao,
  • Xiaoxiong Pan,
  • Ji Wu
Xiaofeng Zhang
The First Affiliated Hospital of Guangxi Medical University

Corresponding Author:[email protected]

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Chunting Liang
The First Affiliated Hospital of Guangxi Medical University
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Jingtao Li
The First Affiliated Hospital of Guangxi Medical University
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Decai Zeng
The First Affiliated Hospital of Guangxi Medical University
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Yanfen Zhong
The First Affiliated Hospital of Guangxi Medical University
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Tongtong Huang
The First Affiliated Hospital of Guangxi Medical University
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Yingying Mo
The First Affiliated Hospital of Guangxi Medical University
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Huaqing Rao
The First Affiliated Hospital of Guangxi Medical University
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Xiaoxiong Pan
The First Affiliated Hospital of Guangxi Medical University
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Ji Wu
The First Affiliated Hospital of Guangxi Medical University
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Abstract

Objectives: Pulmonary artery pressure measurement is key to verifying the success of a rat model of pulmonary hypertension(PH). Herein, a novel method for measuring pulmonary artery pressure through high-frequency ultrasound-guided transthoracic puncture was demonstrated in rats. The efficacy and time of this novel method were also discussed. Methods: A single subcutaneous injection of monocrotaline was used to establish a rat model of PH. The tip of that puncture cannula was maintained at a certain angle after the needle core was removed through heat shaping method. In the short-axis section of the parasternal aorta, in-plane real-time guided trocar puncture of the right ventricular outflow tract was performed. The external pressure sensor was used to record the real-time waveform, right ventricular systolic pressure, pulmonary artery systolic pressure, and diastolic pressure. Results: The success rates of using this novel method in the model group and the control group were 88.5% and 86.7%, respectively, and the puncture pressure measurement times were 164 ± 31s and 235 ± 50s, respectively. The model group’s right ventricular systolic blood pressure, pulmonary systolic blood pressure, and diastolic blood pressure were higher than those of the control group. Conclusions: The modified method for trocar is helpful for accurately positioning pulmonary artery manometry. The method described in this paper has a high success rate and short operation time. It has a broad application prospect in verifying rat PH model and pulmonary artery pressure monitoring.
28 Sep 2022Published in Frontiers in Cardiovascular Medicine volume 9. 10.3389/fcvm.2022.995728