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Characterization of the major allergen, Que ac 1, from sawtooth oak pollen
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  • Kyoung Yong Jeong,
  • Jong Sun Lee,
  • Ji Eun Yuk,
  • Jong Han Park,
  • Fatima Ferreira-Briza,
  • Min Kyu Sang,
  • Yong Seok Lee,
  • Lothar Vogel,
  • Kyung Hee Park,
  • Jung-Won Park
Kyoung Yong Jeong
Yonsei University College of Medicine

Corresponding Author:[email protected]

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Jong Sun Lee
Yonsei University College of Medicine
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Ji Eun Yuk
Yonsei University College of Medicine
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Jong Han Park
Yonsei University College of Medicine
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Fatima Ferreira-Briza
University of Salzburg
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Min Kyu Sang
Soonchunhyang University
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Yong Seok Lee
Soonchunhyang University
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Lothar Vogel
Paul-Ehrlich-Insitut
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Kyung Hee Park
Yonsei University College of Medicine
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Jung-Won Park
Yonsei University College of Medicine
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Abstract

Background: Sawtooth oak is a potent cause of tree pollinosis in Korea. However, molecular characteristics of the major allergen have not been elucidated. Objective: The study was to perform biochemical and allergenic characterization from sawtooth oak. Methods: Native Que ac 1 was purified and analyzed by 2D gel electrophoresis and LC-coupled ESI-MS/MS analysis. Polymorphisms of Que ac 1 were examined by RT-PCR, and a recombinant allergen of the major isoform was produced. IgE reactivities of the native and recombinant allergens were compared by ELISA. Results: At least 12 isoforms of Que ac 1 were identified by proteomic analysis, and 22 isoforms including two isoallergens were identified by RT-PCR. A major isoform Que ac 1.0101 (13.3%, 10/75) shared the lowest homology to Bet v 1 (58.1%) and the highest homology to Que r 1 (83.0%). Recombinant Que ac 1.0101, which exhibited a similar CD spectrum with native Que ac 1, was recognized by serum IgE from 91.3% of Korean oak pollinosis patients. Recombinant Que ac 1 inhibited 67.2% of IgE reactivity to sawtooth pollen extract, but native Que ac 1 and recombinant Bet v 1 inhibited 88.7% and 57.6% of the IgE reactivity, respectively. No difference in allergen potential was shown between Que ac 1 and Bet v 1 by basophil mediator release assay. Conclusion: Molecular properties of the major allergen Que ac 1 were characterized. Que ac 1 seems to be an allergen of regional importance homologous to Bet v 1 and may be useful for better diagnostics of pollinosis.
Aug 2021Published in Allergy volume 76 issue 8 on pages 2626-2629. 10.1111/all.14875