To find out the comprehensive nursing regulation against COVID-19 epidemic in hematology department. Normalized regulation preventing epidemic was set up by improving management of accompany nursing and optimizing the process of admitting to hospital, which include special nursing measures preventing hematology patients with fever during the epidemic; dual quarantine methods for patients with low leukocyte; blood catheter nursing; improving nursing for patients with anemia or low blood platelet. All staff in our department were not infected by COVID-19, and no patients were confirmed, quality of nursing were excellent,100% satisfaction were got both in the staff and patients, the process of admitting and routine work in department were normally running. Awareness of risk is essentially for nurse who practice frontline nursing in hematology ward especially facing the outbreak of epidemic, we should immediately take action against the pandemic to reduce the infection and alleviate the loss.