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Phylogenetic and pathogenicity analysis of a novel lineage of caprine parainfluenza virus type 3
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  • Yanhua Ma,
  • Yan Wang,
  • Xiaohui Zan,
  • Youzhi Wu,
  • Jialei WANG,
  • Gairu Li,
  • chunxia Chai,
  • Cun Fu,
  • Shirong Wang,
  • Hua Yin,
  • Wei Wang
Yanhua Ma
Inner Mongolia University School of Life Science
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Yan Wang
Inner Mongolia University School of Life Science
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Xiaohui Zan
Inner Mongolia University School of Life Science
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Youzhi Wu
Inner Mongolia University School of Life Science
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Jialei WANG
Inner Mongolia University School of Life Science
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Gairu Li
Nanjing Agricultural University
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chunxia Chai
Inner Mongolia University School of Life Science
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Cun Fu
Inner Mongolia University School of Life Science
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Shirong Wang
Inner Mongolia University School of Life Science
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Hua Yin
Inner Mongolia University School of Life Science
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Wei Wang
Inner Mongolia University
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Abstract

Caprine parainfluenza virus type 3 (CPIV3) was first identified in goats named JS2013 in China. In 2019, a sheep herd broke a disease with respiratory disease in Hebei province, China. In order to confirm the pathogen of the disease, the nasal swabs, stool swabs and blood samples were collected from the sheep. Virus isolation was performed on MDBK cells and identification was conducted by RT-PCR. The complete genome of the isolate was sequenced and phylogenetic analyzed. In order to evaluate the pathogenicity of the virus, five seronegative sheep were experimental infected with the virus suspension. The phylogenetic analyses based on the complete genome and the M gene indicated that the isolate strain was distinguished distinct from previously reported CPIV3 lineage of JS2013. The virus-inoculated sheep displayed the syndrome with depression, cough, and fever. Virus shedding were detected by RT-PCR from nasal swabs. All infected showed virus shedding during 2 - 21dpi and viremia could be detected in serum samples. Gross pathological assessment of sheep in infected group showed gross lesion in the lungs. Histopathological observation results indicated that lungs had mild to moderate interstitial pneumonia , with thickened alveolar walls, decreased alveolar space, and increased amounts of inflammatory cells infiltration. This is the first report of pathogenicity of the novel lineage of sheep-derived CPIV3. The results would be helpful for further studies on the prevention and control strategies for CPIV3 infections in goat and sheep.
May 2021Published in Microbial Pathogenesis volume 154 on pages 104854. 10.1016/j.micpath.2021.104854