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Molecular Epidemiological Survey of Canine Parvovirus Circulating in China from 2014 to 2019
  • Bixia Chen,
  • Xiaohui Zhang,
  • Endong Bao
Bixia Chen
Nanjing Agricultural University
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Xiaohui Zhang
Nanjing Agricultural University
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Endong Bao
Nanjing Agricultural University
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Abstract

The global distribution of canine parvovirus (CPV-2), derived from a closely related carnivore parvovirus, has caused a considerable threat to the dog population. The virus continuously underwent genetic evolution, giving rise to several variants. To investigate the prevalence of Chinese CPV-2 strains in recent years, a total of 25 CPV-2 strains were isolated from 33 canine samples collected from 2018 to 2020, and then sequenced and analyzed. Two variants, New CPV-2a and CPV-2c were identified. Contrary to previous reports, the CPV-2c variant has gained an epidemiological advantage over the New CPV-2a variant in China. To make up for the relatively limited sample, 683 Chinese CPV-2 records identified between 2014 to 2019 were retrieved from Genbank and associated publications, whose result further supported our finding. That should be caught concern since the CPV-2c variant has been frequently related to vaccine failure in adult dogs. VP2 protein sequences analysis revealed several amino acid substitutions, including Ala5Gly, Pro13Ser, Phe267Tyr, Tyr324Ile, Gln370Arg, Thr440Ala, and Lys570Arg. Phylogenetic analysis indicated a close relationship between Chinese strains with Asian strains, suggesting the mutual transmission between Asian countries. Furthermore, the intercontinental transmission should be a cause for concern. Surprisingly, two feline panleukopenia (FPLV) strains with Ile101Thr mutation in VP2 protein were also successfully isolated from canine fecal samples, which was considered incapable of infecting dogs. This study clarified the epidemic characteristics of Chinese CPV-2 strains between 2014 and 2019, offering a reference for epidemic control. Besides, the detection of FPLV in canine samples may provide information for future studies on the evolution of carnivore parvovirus.
11 May 2021Published in Pathogens volume 10 issue 5 on pages 588. 10.3390/pathogens10050588