LIFU-augmented Multifunctional Nanoparticles for Integrating Ultrasound
Imaging and Synergistic Therapy of Metastatic Breast Cancer
Abstract
The metastasis of breast cancer is believed to have a negative effect on
its prognosis. Benefiting from the remarkable deep-penetrating and
non-invasive characteristics, sonodynamic therapy (SDT) demonstrates a
whole series of potential leading to cancer treatment. To relieve the
limitation of monotherapy, a multifunctional nanoplatform has been
explored to realize the synergistic treatment efficiency. Herein, we
establish a novel multifunctional nano-system which encapsulates chlorin
e6 (Ce6, for SDT), perfluoropentane (PFP, for ultrasound imaging), and
docetaxel (DTX, for chemotherapy) in a well-designed PLGA core-shell
structure. The synergistic nanoparticles (CPDP NPs) featured with
excellent biocompatibility and stability which primarily enables its
further application. Upon low intensity focused ultrasound (LIFU)
irradiation, the enhanced ultrasound imaging could be revealed both in
vitro and in vivo. More importantly, combined with LIFU, the
nanoparticle exhibits intriguing antitumor capability through Ce6
induced cytotoxic reactive oxygen species (ROS) as well as DTX releasing
to generate a concerted therapeutic efficiency. Furthermore, this
treating strategy actives a strong anti-metastasis capability by which
lung metastatic nodules have been significantly reduced. The results
indicate that the SDT-oriented nanoplatform combined with chemotherapy
could be provided as a promising approach in elevating effective
synergistic therapy and suppressing lung metastasis of breast cancer.