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Aberrant methylation of aging-related genes in asthma
  • +12
  • Yu Yang,
  • Lin Yuan,
  • Ming Yang,
  • Xizi Du,
  • Ling Qin,
  • Leyuan Wang,
  • Kai Zhou,
  • Mengping Wu,
  • Ruoxi He,
  • Juntao Feng,
  • Yang Xiang,
  • Xiangping Qu,
  • Huijun Liu,
  • Xiaoqun Qin,
  • Liu chi
Yu Yang
Central South University

Corresponding Author:[email protected]

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Lin Yuan
Central South University
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Ming Yang
Newcastle University
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Xizi Du
Central South University
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Ling Qin
Central South University
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Leyuan Wang
Central South University
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Kai Zhou
Central South University
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Mengping Wu
Central South University
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Ruoxi He
Central South University
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Juntao Feng
Central South University
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Yang Xiang
Central South University
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Xiangping Qu
Central South University
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Huijun Liu
Central South University
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Xiaoqun Qin
Central South University
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Liu chi
Central South University
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Abstract

Background: Asthma is a complex pulmonary inflammatory disease which is common among older adults.Aging-related alterations have also been found in structural cells and immune cells of asthma patients. Of note, DNA methylation have been proven to be play a critical mechanism for age-related gene expression changes However, the methylation changes of aging-related genes in asthma patients is still obscure. Methods: First, changes in DNAm and gene expression were detected with multiple targeted bisulfite enrichment sequencing and qPCR in peripheral blood of 51 healthy controls and 55 asthmatic patients. Secondly, the correlation between the DNAm levels of specific altered CpG sites and the pulmonary function indicators of asthma patients was evaluated. Lastly, ROC curve and PCA were used to identify the feasibility of the candidate CpG sites as biomarkers for asthma. Results: Compared with HCs, there was a differential mRNA expression for 9 aging-related gene in peripheral blood of asthma patients. Besides, the methylation level of the 9 aging-related genes were also altered, and a total of 68 CpG sites were associated with the severity of asthma. Moreover, ROC curve and PCA analysis showed that the candidate differential methylation sites can be used as potential biomarkers for asthma. Conclusions: In summary, this study confirmed the differentially expressed mRNA and aberrant DNAm level of aging-related genes in asthma patients. The differential DMSs are associated with the clinical evaluation indicators of asthma, which indicate the involvement of aging-related genes in the pathogenesis of asthma and provide some new possible biomarker for asthma.
25 May 2021Published in Frontiers in Molecular Biosciences volume 8. 10.3389/fmolb.2021.655285