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Lineage and ‘role’ in integrative taxonomy of a heterotrophic orchid complex
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  • Craig Barrett,
  • Mathilda Santee,
  • Nicole Fama,
  • John Freudenstein,
  • Sandra Simon,
  • Brandon Sinn
Craig Barrett
West Virginia University

Corresponding Author:[email protected]

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Mathilda Santee
West Virginia University
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Nicole Fama
West Virginia University
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John Freudenstein
The Ohio State University
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Sandra Simon
West Virginia Insitute of Technology
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Brandon Sinn
University of Latvia
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Lineage-based species definitions applying coalescent approaches to species delimitation have become increasingly popular. Yet, the application of these methods and the recognition of lineage-only definitions have recently been questioned. Species delimitation criteria that explicitly consider both lineages and evidence for ecological ‘role’ shifts provide an opportunity to incorporate ecologically meaningful data from multiple sources in studies of species boundaries. Here, such criteria were applied to a problematic group of mycoheterotrophic orchids, the Corallorhiza striata complex, analyzing genomic, morphological, phenological, reproductive-mode, niche, and fungal host data. A recently developed method for generating genomic polymorphism data–ISSRseq–demonstrates evidence for four distinct lineages, including a previously unidentified lineage in the Coast Ranges and Cascades of California and Oregon, USA. There is divergence in morphology, phenology, reproductive mode, and fungal associates among the four lineages. Integrative analyses, conducted in population assignment and redundancy analysis frameworks, provide evidence of distinct genomic lineages and a similar pattern of divergence in the ‘extended’ data, albeit with weaker signal. However, none of the ‘extended’ datasets fully satisfy the condition of a significant ‘role’ shift, which requires evidence of fixed differences. The four lineages identified in the current study are recognized at the level of variety, short of comprising different species. This study represents the most comprehensive application of ‘lineage+role’ to date and illustrates the advantages of such an approach.
09 Mar 2022Submitted to Molecular Ecology
11 Mar 2022Submission Checks Completed
11 Mar 2022Assigned to Editor
20 Mar 2022Reviewer(s) Assigned
19 Apr 2022Review(s) Completed, Editorial Evaluation Pending
25 Apr 2022Editorial Decision: Revise Minor
09 May 2022Review(s) Completed, Editorial Evaluation Pending
09 May 20221st Revision Received
16 May 2022Reviewer(s) Assigned
13 Jun 2022Editorial Decision: Revise Minor
06 Jul 2022Review(s) Completed, Editorial Evaluation Pending
06 Jul 20222nd Revision Received
11 Jul 2022Editorial Decision: Accept
Sep 2022Published in Molecular Ecology volume 31 issue 18 on pages 4762-4781. 10.1111/mec.16617