Orf is an acute and highly contracted human and animal infection caused
by orf virus (ORFV), which mainly affects sheep and goats, especially
young animals, who have close contact with sick animals. Clinically, in
secondary infection cases of orf, opportunistic or conditional pathogens
such as Staphylococcus aureus (S. aureus) are often detected. The S.
aureus TRAP gene product is reportedly protective against bacterial
infection. For joint control of ORFV and S. aureus, the research
direction of delivering a TRAP gene vaccine against S. aureus infection
using the ORFV live vector is proposed. Here, we used CRISPR/Cas9
technology to edit vascular endothelial growth factor E of ORFV (VEGF-V)
and introduced the TRAP gene of S. aureus into the terminus of the ORFV
genome to promote TRAP expression in infected keratinocytes. The
construction and experimental verification of recombinant ORFV
(ORFV-V/TRAP) will provide a reference for in-depth studies on the
prevention and control of orf and S. aureus disease.