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Metabolism-mediated mechanisms underpin the differential stomatal speediness regulation among ferns and angiosperms
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  • Silvio Cândido‐Sobrinho,
  • Valéria Lima,
  • Francisco Freire,
  • Leonardo de Souza,
  • Jorge Gago,
  • Alisdair R. Fernie,
  • Danilo Daloso
Silvio Cândido‐Sobrinho
Universidade Federal do Ceara

Corresponding Author:[email protected]

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Valéria Lima
Universidade Federal do Ceara
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Francisco Freire
Universidade Federal do Ceara
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Leonardo de Souza
Max-Planck-Institut fur molekulare Pflanzenphysiologie
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Jorge Gago
Universitat de les Illes Balears
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Alisdair R. Fernie
Max-Planck Institute for Molecular Plant Physiology
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Danilo Daloso
Universidade Federal do Ceara
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Recent results suggest that metabolism-mediated stomatal closure mechanisms are important to regulate differentially the stomatal speediness between ferns and angiosperms. However, evidence directly linking mesophyll metabolism and the slower stomatal conductance (gs) in ferns is missing. Here we investigated the effect of exogenous application of abscisic acid (ABA), sucrose and mannitol on gs kinetics and carried out a metabolic fingerprinting analysis of ferns and angiosperms leaves harvested throughout a diel course. Ferns stomata did not respond to ABA in the time period analysed. No differences in the relative decrease in gs was observed between ferns and the angiosperm following provision of sucrose or mannitol. However, ferns have slower gs responses to these compounds than angiosperms. Metabolomics analysis highlights that ferns have higher accumulation of secondary rather than primary metabolites throughout the diel course, with the opposite being observed in angiosperms. Our results indicate that metabolism-mediated stomatal closure mechanism is conserved among ferns and angiosperms and that the slower stomatal closure in ferns is associated to a reduced capacity to respond to mesophyll-derived sucrose and to a higher carbon allocation toward secondary metabolism, which likely modulates both photosynthesis-stomatal movements and growth-stress tolerance trade-offs.
06 Oct 2021Submitted to Plant, Cell & Environment
06 Oct 2021Submission Checks Completed
06 Oct 2021Assigned to Editor
10 Oct 2021Reviewer(s) Assigned
03 Nov 2021Review(s) Completed, Editorial Evaluation Pending
03 Nov 2021Editorial Decision: Revise Minor
11 Nov 20211st Revision Received
12 Nov 2021Submission Checks Completed
12 Nov 2021Assigned to Editor
15 Nov 2021Review(s) Completed, Editorial Evaluation Pending
15 Nov 2021Editorial Decision: Accept
Feb 2022Published in Plant, Cell & Environment volume 45 issue 2 on pages 296-311. 10.1111/pce.14232