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Amplicon_sorter: a tool for reference-free amplicon sorting based on sequence similarity and for building consensus sequences
  • Andy Vierstraete,
  • Bart Braeckman
Andy Vierstraete
Ghent University
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Bart Braeckman
Ghent University
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Abstract

Oxford Nanopore Technologies (ONT) is a third-generation sequencing technology that is gaining popularity in ecological research for its portable and low-cost sequencing possibilities. Although the technology is primarily developed for long-read sequencing, it can also be applied to sequence amplicons. The downside of ONT is the low quality of the raw reads. Hence, generating a high-quality consensus sequence is still a challenge. We present Amplicon_sorter, a tool for reference-free sorting of ONT sequenced amplicons based on their similarity in sequence and length and for building solid consensus sequences.
16 Sep 2021Submitted to Ecology and Evolution
17 Sep 2021Submission Checks Completed
17 Sep 2021Assigned to Editor
08 Oct 2021Reviewer(s) Assigned
22 Oct 2021Review(s) Completed, Editorial Evaluation Pending
26 Nov 2021Editorial Decision: Revise Minor
05 Jan 20221st Revision Received
05 Jan 2022Assigned to Editor
05 Jan 2022Submission Checks Completed
05 Jan 2022Review(s) Completed, Editorial Evaluation Pending
11 Jan 2022Editorial Decision: Revise Minor
12 Jan 20222nd Revision Received
12 Jan 2022Submission Checks Completed
12 Jan 2022Assigned to Editor
12 Jan 2022Review(s) Completed, Editorial Evaluation Pending
18 Jan 2022Editorial Decision: Accept
Mar 2022Published in Ecology and Evolution volume 12 issue 3. 10.1002/ece3.8603