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CYP1A expression in freshwater fish of western New York as an indicator of pollution levels
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  • Rebecca Williams,
  • Teri Koetsier,
  • Seema Johnson,
  • Kayla Miller,
  • Theresa Taggart,
  • Amber Dinchman
Rebecca Williams
The College of Wooster

Corresponding Author:[email protected]

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Teri Koetsier
Houghton College
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Seema Johnson
Houghton College
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Kayla Miller
Houghton College
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Theresa Taggart
Houghton College
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Amber Dinchman
The College of Wooster
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Various species of freshwater fish regulate the expression of certain proteins in response to environmental contamination. Previous research has shown that CYP1A expression increases in response to contaminant levels, and can result in increased tumor formation. Fish in contaminated environments would thus benefit by downregulating the expression of CYP1A to reduce tumor prevalence as an adaptive strategy. Alternatively, regulation of the CYP1A protein in fish can serve as a bioindicator of the pollution level of an environment. This study evaluated CYP1A expression in twelve different species of freshwater fish from seven bodies of water throughout western NY including Cuba Lake, Genesee River, Hanging Bog, Love Canal, Moss Lake, Rushford Lake and Tifft Nature Preserve. Western blot analysis was used to measure CYP1A expression as a marker of site pollution and potential fish population adaptation. It was hypothesized that low CYP1A expression at a site with known contamination would suggest signs of adaptation to pollution levels present. Furthermore, if at least one sample from a species showed CYP1A expression, then the CYP1A antibody (Caymen Chemical, USA; 173132) had compatibility with that species, eliminating falsely suspected adaptation. The results from this study suggest possible adaptation of fish may be occurring in the polluted Tifft Nature Preserve and Genesee River. In contrast, CYP1A expression in fish from Cuba Lake, Hanging Bog, Love Canal, Moss Lake, and Rushford Lake appear to represent known pollution levels and adaptation is not likely occurring. Results from this study are preliminary and next steps include collection and analysis of sediment to provide a stronger correlation between pollution at sites and CYP1A expression.
02 Aug 2021Submitted to Ecology and Evolution
05 Aug 2021Submission Checks Completed
05 Aug 2021Assigned to Editor
11 Aug 2021Reviewer(s) Assigned
02 Nov 2021Review(s) Completed, Editorial Evaluation Pending
12 Nov 2021Editorial Decision: Revise Minor
14 Dec 20211st Revision Received
14 Dec 2021Submission Checks Completed
14 Dec 2021Assigned to Editor
14 Dec 2021Review(s) Completed, Editorial Evaluation Pending
17 Dec 2021Editorial Decision: Accept
Jan 2022Published in Ecology and Evolution volume 12 issue 1. 10.1002/ece3.8526