Mad7: An IP friendly CRISPR enzyme
- Johan Blatt Rojek
, - Yogesh Basavaraju,
- Saranya Nallapareddy,
- Roland Baumgartner,
- Sanne Schoffelen,
- Lasse Pedersen
Johan Blatt Rojek
Danmarks Tekniske Universitet
Corresponding Author:johroj@biosustain.dtu.dk
Author ProfileRoland Baumgartner
Danmarks Tekniske Universitet The Novo Nordisk Foundation Center for Biosustainability
Author ProfileAbstract
Mad7 is a CRISPR enzyme, similar to Cas9 and Cas12a, which is of great
interest to industry and academia due to its permissive licensing
agreement, which states that everyone is free to use it but not
distribute it commercially. This paper demonstrates that Mad7 genome
editing works in CHO cells and provides the CHO engineering community
with the computational tools needed to generate Mad7 compatible gRNAs
against the CHO genome.
