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Mad7: An IP friendly CRISPR enzyme
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  • Johan Blatt Rojek,
  • Yogesh Basavaraju,
  • Saranya Nallapareddy,
  • Roland Baumgartner,
  • Sanne Schoffelen,
  • Lasse Pedersen
Johan Blatt Rojek
Danmarks Tekniske Universitet
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Yogesh Basavaraju
Danmarks Tekniske Universitet
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Saranya Nallapareddy
Danmarks Tekniske Universitet
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Roland Baumgartner
Danmarks Tekniske Universitet The Novo Nordisk Foundation Center for Biosustainability
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Sanne Schoffelen
Danmarks Tekniske Universitet
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Lasse Pedersen
Danmarks Tekniske Universitet
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Abstract

Mad7 is a CRISPR enzyme, similar to Cas9 and Cas12a, which is of great interest to industry and academia due to its permissive licensing agreement, which states that everyone is free to use it but not distribute it commercially. This paper demonstrates that Mad7 genome editing works in CHO cells and provides the CHO engineering community with the computational tools needed to generate Mad7 compatible gRNAs against the CHO genome.

Peer review status:UNDER REVIEW

02 Aug 2021Submitted to Biotechnology Journal
03 Aug 2021Assigned to Editor
03 Aug 2021Submission Checks Completed
10 Aug 2021Reviewer(s) Assigned