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Prediction and characterization of the T cell epitopes for the major soybean protein allergens using bioinformatics approaches
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  • Fanlin Zhou,
  • Shudong He,
  • Yongfei Wang,
  • Hanju Sun,
  • Yi Zhang
Fanlin Zhou
Hefei University of Technology
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Shudong He
Hefei University of Technology
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Yongfei Wang
Hefei University of Technology
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Hanju Sun
Hefei University of Technology
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Yi Zhang
McGill University
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Protein allergens is a health risk for consumption of soybeans. To understand allerginicity mechanism, T cell epitopes of 7 soybean allergens were predicted and screened by abilities to induce cytokine interleukin 4. The relationships among amino acid composition, properties, allergenicity and pepsin hydrolysis sites were analyzed. Among the 138 T cell epitopes identified, YIKDVFRVIPSEVLS, KDVFRVIPSEVLSNS, DVFRVIPSEVLSNSY of Gly m 6.0501 (P04347), and AKADALFKAIEAYLL, ADALFKAIEAYLLAH of Gly m 4.0101 (P26987) were the most possible epitope candidates. In T cell epitopes pattern, the frequencies of amino acids Q, D, E, P and G decreased, while F, I, N, V, K and H increased. Hydrophobic residues at positions p1 and p2 and positively charged residues in positions p13 might contribute to allergenicity. Most of epitopes could be hydrolyzed by pepsin into small polypeptides within 12 residues length, and the anti-digestive epitope regions contained I, V, S, N, and Q residues. T cell epitopes EEQRQQEGVIVELSK from Gly m 5.03 (P25974) showed resistantence to pepsin hydrolysis and would cause a higher Th2 cell response. This research provides basis for the development of hypoallergenic soybean products in the soybean industry as well as for the immunotherapy design for protein allergy.

Peer review status:ACCEPTED

19 Jul 2021Submitted to PROTEINS: Structure, Function, and Bioinformatics
20 Jul 2021Submission Checks Completed
20 Jul 2021Assigned to Editor
22 Jul 2021Reviewer(s) Assigned
06 Aug 2021Review(s) Completed, Editorial Evaluation Pending
08 Aug 2021Editorial Decision: Revise Minor
11 Aug 20211st Revision Received
16 Aug 2021Submission Checks Completed
16 Aug 2021Assigned to Editor
16 Aug 2021Reviewer(s) Assigned
22 Aug 2021Review(s) Completed, Editorial Evaluation Pending
30 Aug 2021Editorial Decision: Accept