Abstract Background It is not clear if there is a inaccurate effect of obesity and pneumoperitoneum on non-invasive monitoring parameters Objectives To determine the effects of CO2 pneumoperitoneum and desufflation on non-invasive monitoring variables between obese and non-obese patients undergoing laparoscopic surgery. Methods Sixty patients were included in the study who underwent laparoscopic cholecystectomy between February 2019-November 2019. After anesthesia induction, systolic and diastolic blood pressures, heart rate(HR), operation and pneumoperitoneum durations, PVI and SpHg data were recorded. Results The patients were divided into two groups. There was no statistically significant difference between the groups in terms of chronic diseases, operation and pneumoperitoneum durations. Statistically no significant difference was found between the groups for PVI and SpHb values. In comparison of PVI measurement values according to T0, a significant difference was found in T3,T4 and T5 in Group 2 compared to T0 (p=0.010;0.012;0.041). In comparison of SpHb measurement values according to T0, a statistically significant difference was found in T1,T2 and T3 in Group 2 compared to T0 (p=0.008;0.010;0.037). In comparison to T5, there was a significant difference in T1,T2 and T3 in Group 2 (p=0.023;0.005;0.006). Conclusion In obese people, pneumoperitonium can lead to inaccuracies in monitoring PVI and SpHb. Although these two parameters are very valuable in intraoperative follow-up, they should be used carefully during laparoscopic procedures in obese patients. If these parameters (PVI and SpHb) are considered to be used in bariatric surgeries, this information should be considered.