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Effect of different cell disruption methods on lipid yield of Schizochytrium sp.
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  • Mustafa Hac İsa,
  • Cansu Metin,
  • Ertan Ercan,
  • Yunus Alparslan
Mustafa Hac İsa
Muğla Sıtkı Koçman University
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Cansu Metin
Muğla Sıtkı Koçman University
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Ertan Ercan
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Yunus Alparslan
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Abstract

In this study, it was investigated to increase the lipid yield of the microalgae Schizochytrium sp., by applying different cell disruption methods. Therefore, acid treatment with HCl, osmotic shock, enzyme applications and ultrasonic homogenizer were tried in this algae species combined with the Bligh and Dyer and Soxhlet methods as an alternative to classical lipid extraction methods. As a result of the study, the highest lipid value (21.72 ± 0.74%) was obtained in enzyme application with Bligh and Dyer method (BDE). The cell disruption processes increased the lipid yield compared to the control groups. The highest PUFA DHA was found in the range of 4.58 ± 2.44-19.25 ± 0.09%, and the highest value was observed in the BDE group. Highest SFA was palmitic acid. Effective results were observed in the Bligh and Dyer applied groups in terms of both total lipid and total fatty acids. In cell disruption methods, particularly in enzyme and HCl extraction, good results were obtained in terms of fatty acids. The highest total fatty acids and the highest lipid content were detected in the Bligh and Dyer enzyme (BDE). Enzyme applications are also advantageous because of being environmentally friendly. Lipid health indices such as n-6/n-3, PUFA/SFA, Atherogenicity index (AI), Thrombogenicity index (TI) and hypocholesterolemic/hypercholesterolemic ratios (HH) were almost favorable. With this study, an appropriate lipid extraction methods were determined to provide an economical and environmental friendly suggestion for future studies to be used in areas such as food, feed and cosmetics.

Peer review status:IN REVISION

04 Jun 2021Submitted to Journal of the American Oil Chemists' Society
04 Jun 2021Submission Checks Completed
04 Jun 2021Assigned to Editor
17 Jun 2021Reviewer(s) Assigned
12 Jul 2021Review(s) Completed, Editorial Evaluation Pending
30 Jul 2021Editorial Decision: Revise Major
18 Aug 20211st Revision Received
18 Aug 2021Submission Checks Completed
18 Aug 2021Assigned to Editor
18 Aug 2021Reviewer(s) Assigned
06 Sep 2021Review(s) Completed, Editorial Evaluation Pending
16 Sep 2021Editorial Decision: Revise Minor