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Airbrushed Nanofibrous Membranes to Control Stem Cell Infiltration In 3D Printed Scaffolds
  • +4
  • Chya-Yan Liaw,
  • Shawn Huynh,
  • Christina Gedeon,
  • Shen Ji,
  • Caroline D’souza,
  • Alperen Abaci,
  • Murat Guvendiren
Chya-Yan Liaw
New Jersey Institute of Technology
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Shawn Huynh
New Jersey Institute of Technology
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Christina Gedeon
New Jersey Institute of Technology
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Shen Ji
New Jersey Institute of Technology
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Caroline D’souza
New Jersey Institute of Technology
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Alperen Abaci
New Jersey Institute of Technology
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Murat Guvendiren
New Jersey Institute of Technology
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Abstract

Extrusion-based 3D printing of polymeric biomaterials has emerged as a promising approach for the fabrication of complex tissue engineering constructs. However, the large pore and feature size lead to low cell seeding efficiency and limited control of spatial distribution of cells within the scaffolds. We developed hybrid scaffolds that are composed of 3D printed layers and airbrushed fibrous membranes. Airbrushing time was adjusted to fabricate low (L), medium (M), and high (H) density membranes to effectively control stem cell infiltration. When two distinct populations of stem cells were seeded from top or bottom of the scaffolds, scaffolds composed of LLL membranes showed gradual mixing of the cells with depth whereas LHL membranes led to two distinct regions of cells separated by the H membrane. Our results demonstrate that fibrous membranes incorporated within 3D printed layers enable user-defined and spatially controlled cell compositions within hybrid scaffolds.

Peer review status:UNDER REVIEW

22 May 2021Submitted to AIChE Journal
31 May 2021Submission Checks Completed
31 May 2021Assigned to Editor
13 Jun 2021Reviewer(s) Assigned
15 Aug 2021Editorial Decision: Revise Major
31 Aug 20211st Revision Received
31 Aug 2021Submission Checks Completed
31 Aug 2021Assigned to Editor
02 Sep 2021Reviewer(s) Assigned