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Macroparticle-enhanced cultivation of Lentzea aerocolonigenes: Variation of mechanical stress and combination with lecithin supplementation for a significantly increased rebeccamycin production
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  • Kathrin Schrinner,
  • Marcel Schrader,
  • Jana Niebusch,
  • Kristin Althof,
  • Friederike Schwarzer,
  • Paul-Frederik Nowka,
  • Anna Dinius,
  • Arno Kwade,
  • Rainer Krull
Kathrin Schrinner
TU Braunschweig
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Marcel Schrader
Technische Universität Braunschweig
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Jana Niebusch
Technische Universität Braunschweig
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Kristin Althof
Technische Universität Braunschweig
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Friederike Schwarzer
Technische Universität Braunschweig
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Paul-Frederik Nowka
Technische Universität Braunschweig
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Anna Dinius
Technische Universität Braunschweig
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Arno Kwade
Technische Universität Braunschweig
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Rainer Krull
TU Braunschweig
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Abstract

The actinomycete Lentzea aerocolonigenes produces the antitumor antibiotic rebeccamycin. In previous studies the rebeccamycin production was significantly increased by the addition of glass beads during cultivation in different diameters between 0.5 – 2 mm and the induced mechanical stress by the glass beads was proposed to be responsible for the increased production. Thus, this study was conducted to be a systematic investigation of different parameters for macroparticle addition, such as bead diameter, concentration and density (glass and ceramic) as well as shaking frequency, for a better understanding of the particle induced stress on L. aerocolonigenes. The induced stress for optimal rebeccamycin production can be estimated by a combination of stress energy and stress frequency. In addition, the macroparticle-enhanced cultivation of L. aerocolonigenes was combined with soy lecithin addition to further increase the rebeccamycin concentration. With 100 g L-1 glass beads in a diameter of 969 µm and 5 g L-1 soy lecithin a concentration of 388 mg L 1 rebeccamycin was reached after 10 days of cultivation, which corresponds to the highest rebeccamycin concentrations achieved in shake flask cultivations of L. aerocolonigenes stated in literature so far.

Peer review status:ACCEPTED

03 Jun 2021Submitted to Biotechnology and Bioengineering
03 Jun 2021Assigned to Editor
03 Jun 2021Submission Checks Completed
12 Jun 2021Reviewer(s) Assigned
19 Jun 2021Review(s) Completed, Editorial Evaluation Pending
19 Jun 2021Editorial Decision: Accept