loading page

The first high-quality chromosomal genome assembly of a medicinal and edible plant Arctium lappa
  • +12
  • Liang Xu,
  • Shengnan Li,
  • Yanyun Yang,
  • Yanping Xing,
  • Zhongren Zhang,
  • Tao Liu,
  • Wuliji Ao,
  • Guihua Bao,
  • Zhilai Zhan,
  • Rong Zhao,
  • Tingting Zhang,
  • Dachuang Zhang,
  • Tingguo Kang,
  • Yueyue Song,
  • Che Bian
Liang Xu

Corresponding Author:861364054@qq.com

Author Profile
Shengnan Li
Liaoning University of Traditional Chinese Medicine - Dalian Campus
Author Profile
Yanyun Yang
Author Profile
Yanping Xing
Author Profile
Zhongren Zhang
Novogene Bioinformatics Institute
Author Profile
Guihua Bao
Author Profile
Zhilai Zhan
Author Profile
Tingting Zhang
Author Profile
Dachuang Zhang
Author Profile
Tingguo Kang
Author Profile
Yueyue Song
Author Profile


Arctium lappa has a long medicinal and edible history with great economic importance. We combined Illumina and PacBio sequences to generate the first high-quality chromosome-level draft genome of A. lappa. The assembled genome is approximately 1.79 Gb with a N50 contig size of 6.88 Mb. Approximately 1.70 Gb (95.4%) of the contig sequences were anchored onto 18 chromosomes using Hi-C data; the scaffold N50 was improved to be 91.64 Mb. Furthermore, we obtained 1.12 Gb (68.46%) of repetitive sequences and 32,771 protein-coding genes; 616 positively selected candidate genes were identified. Additionally, we compared the transcriptomes of A. lappa roots at three different developmental stages and identified 8,943 differentially expressed genes (DEGs) in these tissues. Among candidate genes related to lignan biosynthesis, the following were found to be highly correlated with the accumulation of arctiin: 4-coumarate-CoA ligase (4CL), dirigent protein (DIR), and hydroxycinnamoyl transferase (HCT). These data can be utilized to identify genes related to A. lappa quality or provide a basis for molecular identification and comparative genomics among related species.
01 Mar 2021Submitted to Molecular Ecology Resources
03 May 2021Submission Checks Completed
03 May 2021Assigned to Editor
04 May 2021Reviewer(s) Assigned
27 Jul 2021Review(s) Completed, Editorial Evaluation Pending
28 Jul 2021Editorial Decision: Revise Minor
12 Aug 2021Review(s) Completed, Editorial Evaluation Pending
12 Aug 20211st Revision Received
07 Sep 2021Editorial Decision: Accept