Relationship of E-cadherin, Beta-catenin, N-cadherin, ZEB1 and αSMA as
Epithelial Mesenchymal Transition markers with prognostic factors in
early and advanced stage laryngeal squamous cell carcinomas
Objective: To investigate the relationship between E-cadherin,
Beta-catenin, N-cadherin, ZEB1 and αSMA as eithelial mesenchymal
transformation markers with tumor stage, lymph node metastasis (LNM) and
overall survival (OS) in laryngeal squamous cell carcinomas (LSCC).
Material and method: A total of 100 cases diagnosed with LSCC in our
hospital between 2013-2020 were included in the study. Data about
lymphovascular invasion (LVI), perineural invasion (PNI), necrosis and
LNM were recorded by evaluating hematoxylin- eosin stained slides.
Markers of E-cadherin, beta-catenin, N-cadherin, ZEB1 and αSMA were
applied to the sections prepared from paraffin blocks of tumor samples.
Results: Ninety-five male and five female patients were included in the
study, and 38 of them exited. The average OS time of the cases was 35.8
months. A significant relationship was observed between OS with advanced
tumor stage, presence of LNM and PNI. A significant relationship was
found between increased tumor Zeb1 expression and advanced tumor stage.
In univariate and multivariate analyses, a significant negative
relationship with OS, and increased Zeb1 expression in tumor and tumor
stroma was seen. Any relationship was not observed between E-cadherin,
beta-catenin, N-cadherin and αSMA and OS. , Conclusion Among the EMT
markers we evaluated in our study, it was seen that Zeb1, which is an
EMT transcription factor, is associated with tumor stage, LNM, and OS.
Remarkably, Zeb1 expression observed in tumor stroma was also
significant for OS. Any similar data reported for LSCCs have not been
encountered in the literature, and it was thought that it would be
appropriate to support our findings with further studies to be performed
on this subject.