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DNA barcoding of deep-sea fishes from the northwestern Pacific Ocean: a resource for identifying hidden genetic diversity in deep-sea fishes
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  • Akinori Teramura,
  • Keita Koeda,
  • Hiroshi Senou,
  • Hsuan-Ching Ho,
  • Kiyoshi Kikuchi,
  • Shotaro Hirase
Akinori Teramura
The University of Tokyo

Corresponding Author:[email protected]

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Keita Koeda
Kuroshio Biological Research Foundation
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Hiroshi Senou
Kanagawa Prefectural Museum of Natural History
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Hsuan-Ching Ho
National Museum of Marine Biology and Aquarium Exhibition Division
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Kiyoshi Kikuchi
The University of Tokyo
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Shotaro Hirase
The University of Tokyo
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COI-based DNA barcoding could be an efficient tool for species identification of deep-sea fishes and could lead to the discovery of cryptic species diversity. However, the availability of reference sequences of deep-sea fishes for DNA barcoding is limited, especially for fishes in the northwestern Pacific Ocean. In this study, we performed DNA barcoding of mesopelagic and demersal fish species on the continental shelf and upper slope, collected from deepwater fisheries around Japan and southern Taiwan, to accumulate the reference sequences of deep-sea fishes in the northwestern Pacific Ocean. Overall, we obtained the COI sequences of 123 species from 50 families. Genetic diversity within each species for which COI sequences were obtained from multiple specimens was examined, and we found that Chimaera phantasma (Chimaeridae), Harpadon microchir (Synodontidae), and Pyramodon ventralis (Carapidae) showed high intraspecific genetic differentiation of more than 2% Kimura two-parameter distance. Moreover, for 19 widespread deep-sea fishes, a comparison between our data and previously acquired COI sequence data suggested a high level (more than 2% Kimura two-parameter distance) of genetic differentiation between the northwestern Pacific Ocean and other oceans in each widespread species. These results suggest that many cryptic species or regional populations have not yet been discovered in deep-sea fishes. Alternatively, genetic differentiation was not found worldwide for six species. These results indicate that many taxonomic and biogeographical issues remain for deep-sea fishes, and our DNA barcoding data would provide better understanding of these issues.
10 Mar 2021Submitted to Molecular Ecology Resources
22 Mar 2021Assigned to Editor
22 Mar 2021Submission Checks Completed
23 Mar 2021Reviewer(s) Assigned
22 May 2021Review(s) Completed, Editorial Evaluation Pending