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Microfluidic Devices for Behavioral Screening of Multiple Zebrafish Larvae: Design Investigation Process
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  • Arezoo Khalili,
  • Ellen van Wijngaarden,
  • khaled Youssef ,
  • Georg Zoidl,
  • Pouya Rezai
Arezoo Khalili
York University

Corresponding Author:[email protected]

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Ellen van Wijngaarden
York University
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khaled Youssef
York University
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Georg Zoidl
York University
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Pouya Rezai
York University
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Microfluidic devices have been introduced for phenotypic screening of zebrafish larvae in both fundamental and pre-clinical research. One of the remaining challenges for the broad use of microfluidic devices is their limited throughput, especially in behavioural assays. Previously, we introduced the tail locomotion of a semi-mobile zebrafish larva evoked on-demand with electric signal in a microfluidic device. Here, we report the lessons learned for increasing the number of specimens from one to four larvae in this device. Multiple parameters including loading and testing time per fish and loading and orientation efficiencies were refined to optimize the performance of modified designs. Simulations of the flow and electric field within the final device provided insight into the flow behavior and functionality of traps when compared to previous single-larva devices. Outcomes led to a new design which decreased the testing time per larva by approximately 60%. Further, loading and orientation efficiencies increased by more than 80%. Critical behavioural parameters such as response duration and tail beat frequency were similar in both single and quadruple-fish devices. The optimized microfluidic device has significant advantages for greater throughput and efficiency when behavioral phenotyping is required in various applications, including chemical testing in toxicology and gene screening.
07 Feb 2021Submitted to Biotechnology Journal
08 Feb 2021Submission Checks Completed
08 Feb 2021Assigned to Editor
11 Feb 2021Reviewer(s) Assigned
08 Jun 2021Editorial Decision: Revise Major
03 Aug 20211st Revision Received
05 Aug 2021Assigned to Editor
05 Aug 2021Submission Checks Completed
05 Aug 2021Reviewer(s) Assigned
18 Aug 2021Editorial Decision: Revise Minor
25 Aug 20212nd Revision Received
26 Aug 2021Assigned to Editor
26 Aug 2021Submission Checks Completed
26 Aug 2021Reviewer(s) Assigned
30 Aug 2021Editorial Decision: Accept