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In Vitro Scolicidal Effects of Sideritis perfoliata Aerial Part Extract against the Protoscoleces of Echinococcus granulosus
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  • TUNCAY Çelik,
  • Muhittin Önderci,
  • Mustafa Pehlivan,
  • Önder Yumrutaş
Adiyaman Universitesi Tip Fakultesi

Corresponding Author:[email protected]

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Muhittin Önderci
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Mustafa Pehlivan
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Önder Yumrutaş
Adiyaman University
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Background: Cystic echinococcosis (CE) is commonly located in the liver and lungs of affected hosts. Surgical management is one of the best choices for the treatment of hydatidosis and using effective scolicidal agents during hydatid surgery is essential to prevent secondary infection. The present study was designed to investigate the in vitro scolicidal activity of methanol extract of Sideritis perfoliata against the protoscoleces of hydatid cysts. Methods: The protoscoleces were collected from slaughtered livestock in Adiyaman and the effect of three concentrations of the aerial part extract of S. perfoliata (0.1mg/ml, 0.2mg/ml, and 0.4mg/ml) was assessed over three different exposure periods. All tests were carried in dublicate. Finally, the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining). Methanol extract of S. perfoliata was assessed by Liquid Chromatography-Mass Spectrometry/Mass Spectrometry (LC-MS/MS). Results: The results showed that the scolicidal effect of this extract at exposure periods of 10, 20, and 30 min was 29.6, 32.5, and 43.6% at concentrations of 0.1mg/ml, 37.8, 50, and 58.1% at concentration of 0.2mg/ml and finally 57.9, 71.8, and 79.1% at concentration of 0.4mg/ml, respectively; indicating that the extract requiring a further time to display a potent protoscolicidal effects. Some phenolic acids such as fumaric acid (260,13mg/L), syringic acid (27,92mg/L) and caffeic acid (26,84mg/L) and a flavonoid, luteolin (11,23 mg/L) were detected in high concentrations. Conclusions: The present study has demonstrated that the methanol extract of S. perfoliata has high scolicidal power in vitro, although the low concentration of plant extract may provide a base for future treatment of hydatid cysts. However, more research on the in vivo efficacy of S. perfoliata extract and its potential side effects is recommended.
07 Dec 2020Submitted to International Journal of Clinical Practice
11 Dec 2020Submission Checks Completed
11 Dec 2020Assigned to Editor
17 Jan 2021Reviewer(s) Assigned
19 Jan 2021Review(s) Completed, Editorial Evaluation Pending
01 Mar 20211st Revision Received
08 Mar 2021Submission Checks Completed
08 Mar 2021Assigned to Editor
26 Apr 2021Reviewer(s) Assigned
27 Apr 2021Review(s) Completed, Editorial Evaluation Pending
06 May 20212nd Revision Received
06 May 2021Submission Checks Completed
06 May 2021Assigned to Editor
06 May 2021Review(s) Completed, Editorial Evaluation Pending
16 May 20213rd Revision Received
18 May 2021Submission Checks Completed
18 May 2021Assigned to Editor
18 May 2021Review(s) Completed, Editorial Evaluation Pending
10 Jun 2021Editorial Decision: Accept