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Characterization of Four Novel H5N6 Avian Influenza Viruses with the Internal Genes from H5N1 and H9N2 Viruses and Experimental Challenge of Chickens Vaccinated with Current Commercially Available H5 Vaccines
  • +11
  • Peng Chen,
  • Pengwei Zhao,
  • Jun Chu,
  • Junda Zhu,
  • Qiuchen Li,
  • Haiyuan Zhao,
  • Yujie Li,
  • Lingxiang Xin,
  • Xiaoyue Yang,
  • Shijie Xie,
  • Changdong Zhu,
  • wenbao Qi,
  • Guanlong Xu,
  • Jinxiang Li
Peng Chen
Laboratory of Viral Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Bethesda, MD 20892

Corresponding Author:[email protected]

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Pengwei Zhao
Department of Biochemistry and Molecular Biology, School of Medicine, Zhejiang University
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Junda Zhu
College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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Qiuchen Li
Key Laboratory of Animal Epidemiology and Zoonosis, Ministry of Agriculture, College of Veterinary Medicine, China Agricultural University, Beijing 100193, China
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Haiyuan Zhao
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Lingxiang Xin
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Xiaoyue Yang
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Shijie Xie
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Changdong Zhu
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Guanlong Xu
China Institute of Veterinary Drug Control
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Jinxiang Li
National Agricultural Science and Technology Center
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Since 2014, highly pathogenic avian influenza H5N6 viruses have been responsible for outbreaks in poultry. In this study, four H5N6 virus strains were isolated from fecal samples of sick white ducks and dead chickens in Shandong in 2019. These H5N6 viruses were triple-reassortant viruses that have not been previously characterized. Their HA genes were derived from the H5 viruses and were closely related to the vaccine strain Re-11. Their NA genes all fell into the N6-like lineage and the internal gene were derived from H5N1 and H9N2 viruses. They all showed high pathogenicity in mice and caused lethal infection with high rates of transmission in chickens. Moreover, the SPF chickens inoculated with the current used vaccine in China were completely protected from these four H5N6 viruses. Our study indicated the necessity of continued surveillance for H5 IAV and the importance of timely update of vaccine strains in poultry industry.
02 Oct 2020Submitted to Transboundary and Emerging Diseases
18 Nov 2020Submission Checks Completed
18 Nov 2020Assigned to Editor
29 Nov 2020Reviewer(s) Assigned
15 Dec 2020Review(s) Completed, Editorial Evaluation Pending
15 Dec 2020Editorial Decision: Revise Minor
23 Dec 20201st Revision Received
11 Mar 2021Assigned to Editor
11 Mar 2021Submission Checks Completed
12 Mar 2021Reviewer(s) Assigned
22 Mar 2021Review(s) Completed, Editorial Evaluation Pending
23 Mar 2021Editorial Decision: Revise Minor
08 Apr 20212nd Revision Received
08 Apr 2021Submission Checks Completed
08 Apr 2021Assigned to Editor
08 Apr 2021Reviewer(s) Assigned
09 Apr 2021Review(s) Completed, Editorial Evaluation Pending
13 Apr 2021Editorial Decision: Accept