LEIDEN V FACTOR AND METHYLEN TETRAHYDROFOLATE REDUCTASE ENZYME GENE
MUTATIONS IN PRIMIGRAVIDAS WITH MISSED ABORTION IN LATE FIRST TRIMESTER:
CROSS-SECTIONAL STUDY
Abstract
ABSTRACT Nowadays , thrombophilia is one of the leading causes of RPL
which has negative side effects due to psychological effect and the high
expenses of investigations and attempts to get normal full term
pregnancy up to ICSI trials in some cases. So as early as possible we
try to diagnose the cause of abortion and treat it to avoid another bad
experience of abortion. Aim of the work: to evaluate the correlation of
factor V Leiden and MTHFR C677T gene mutation in primigravida late first
trimester abortion and to identify candidates for anticoagulation
therapy to improve pregnancy outcome .METHODOLOGY: polymerase chain
reaction (PCR) and reverse-hybridization were used to assess MTHFR and
FV leiden mutations . Results: MTHFR C 677T mutations were 42.5 %.
While factor V leiden gene mutation were 20%. Combined MTHFR C 677T
mutations and factor V leiden was 10 %, normal cases were 27.5%. The
cases without MTHFR C 677Tgene mutations were 47.5% . While The cases
with MTHFR C 677T mutations were 52.5%. Heterozygous MTHFR C 677T
mutations cases were 37.5 % , homozygous cases were 15% .The cases
without Factor V Leiden gene mutations were 70 %, cases with Factor V
Leiden mutations were 30%. All cases with Factor V Leiden mutations
were heterozygous and there was no homozygous cases. Conclusion: MTHFR
mutation is highly expected in late first trimester missed abortions in
primigravida specially the heterotype so significantly affect the
pregnancy adversely while FACTOR V LEIDEN mutation is less likely to be
found.