loading page

Low field NMR Time Domain (TD) Characterization of PUFA-rich Linseed and Fish Oil Emulsions During Thermal Air Oxidation
  • Maysa Resende,
  • Charles Linder,
  • Zeev Wiesman
Maysa Resende

Corresponding Author:[email protected]

Author Profile
Charles Linder
Author Profile
Zeev Wiesman
Author Profile


Linseeds contains high levels of PUFA α-linolenic acid, naturally protected against thermal oxidation by their encapsulation within LS oil bodies by multiple components including antioxidant proteins and mucilage emulsifying agents. By LS grinding, adding of water, adjusting pH, and sonication LS oil bodies emulsions (LSE) can be formed which can also encapsulate externally added PUFAs, to minimize their thermal oxidation, as it does for the intrinsic ALA PUFAs. Fish oil encapsulation into this LSE platform (LSFE) offers the possibility of a nutritive delivery system of the biologically essential PUFA fish oil’s, protected from oxidation, which to date is difficult to achieve. In this study structural and chemical properties LF 1H NMR T1-T2 characterization of LSE and LSFE was used to analyze their stability and changes, under thermal oxidizing conditions. Peak changes in these LF 1H-NMR spectra were correlated with the stability of chemical and physical variables during thermal (55oC for 96 hrs) oxidation. The present study demonstrates the capability of 1H LF-NMR relaxation sensor to monitor the time domain fingerprints of chemical and structural changes of LSE and with co-encapsulated fish oil (LSFE) under thermal autoxidation conditions. The results of the LF-1H NMR analysis are further supported and correlated with conventional peroxide value tests, self-diffusion, droplets size distribution, zeta potential estimation of surface stability under thermal oxidation conditions. The results of this study demonstrate the efficacy of LSE to minimize linseed and encapsulated fish oil PUFA oxidation.
14 Sep 2020Submitted to Journal of the American Oil Chemists' Society
14 Sep 2020Submission Checks Completed
14 Sep 2020Assigned to Editor
05 Oct 2020Reviewer(s) Assigned
15 Nov 2020Review(s) Completed, Editorial Evaluation Pending
04 Dec 2020Editorial Decision: Revise Major
17 Dec 20201st Revision Received
17 Dec 2020Assigned to Editor
17 Dec 2020Submission Checks Completed
18 Dec 2020Reviewer(s) Assigned
01 Jan 2021Review(s) Completed, Editorial Evaluation Pending
12 Jan 2021Editorial Decision: Revise Major
14 Jan 20212nd Revision Received
14 Jan 2021Submission Checks Completed
14 Jan 2021Assigned to Editor
14 Jan 2021Reviewer(s) Assigned
24 Feb 2021Review(s) Completed, Editorial Evaluation Pending
15 Mar 2021Editorial Decision: Accept