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Optimization of a transient antibody expression platform towards high titer and efficiency
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  • Elizabeth Greene,
  • Ninkka Tamot,
  • Daniela Cazacu,
  • Douglas Gebhard,
  • Steven Castellano,
  • Anthony Kronkaitis,
  • Jon Reed,
  • Paul Mason,
  • Lore Florin,
  • Nicholas Rossi,
  • Anthony Lauer,
  • Laura Juckem,
  • Andrew Nixon,
  • Till Wenger,
  • Saurabh Sen
Elizabeth Greene

Corresponding Author:[email protected]

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Ninkka Tamot
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Daniela Cazacu
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Douglas Gebhard
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Steven Castellano
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Anthony Kronkaitis
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Paul Mason
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Lore Florin
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Nicholas Rossi
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Anthony Lauer
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Laura Juckem
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Andrew Nixon
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Till Wenger
Boehringer Ingelheim
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Saurabh Sen
Boehringer Ingelheim Corp USA
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Transient gene expression (TGE) using mammalian cells is an extensively used technology for production of antibodies and recombinant proteins and has been widely adapted by both academic and industrial labs. Chinese Hamster Ovary (CHO) cells have become one of the major work horses for TGE of recombinant antibodies due to their attractive features: post-translational modifications, adaptation to high cell densities, and use of serum-free media. In this study, we describe the optimization of parameters for TGE for antibodies from CHO cells. Through a matrix evaluation of multiple factors including inoculum, transfection conditions, amount and type of DNA used and post-transfection culture conditions, we arrived at an optimized process with higher titer and reduced costs and time, thus increasing the overall efficiency of early antibody material supply. We investigated the amount of coding DNA and the influence of size of the transfection complex on the in vitro efficiency of the transfection. Generation of the transfection complex in serum-free medium leads to the prompt formation of an optimal-sized polyplex, and is independent of the relative amount of coding DNA used for a successful transfection outcome.
29 May 2020Submitted to Biotechnology Journal
03 Jun 2020Submission Checks Completed
03 Jun 2020Assigned to Editor
06 Jun 2020Reviewer(s) Assigned
14 Jul 2020Editorial Decision: Revise Major
09 Oct 20201st Revision Received
10 Oct 2020Assigned to Editor
10 Oct 2020Submission Checks Completed
10 Oct 2020Reviewer(s) Assigned
04 Nov 2020Editorial Decision: Revise Minor
10 Nov 20202nd Revision Received
10 Nov 2020Submission Checks Completed
10 Nov 2020Assigned to Editor
10 Nov 2020Reviewer(s) Assigned
19 Nov 2020Editorial Decision: Accept
Apr 2021Published in Biotechnology Journal volume 16 issue 4 on pages 2000251. 10.1002/biot.202000251