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Benzoate synthesis from glucose or glycerol using engineered Pseudomonas taiwanensis
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  • Maike Otto,
  • Benedikt Wynands,
  • Jan Marienhagen,
  • Lars Blank,
  • Nick Wierckx
Maike Otto
Forschungszentrum Jülich GmbH

Corresponding Author:[email protected]

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Benedikt Wynands
Forschungszentrum Julich GmbH
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Jan Marienhagen
Forschungszentrum Julich GmbH
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Lars Blank
Rheinisch Westfalische Technische Hochschule Aachen
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Nick Wierckx
Forschungszentrum Jülich GmbH
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Benzoic acid is one of the most commonly used food preservatives, but currently exclusively produced in petrochemical processes. In this study, we describe a bio-based production pathway using an engineered strain of Pseudomonas taiwanensis. In a phenylalanine-overproducing strain, we heterologously expressed bacterial, yeast, and plant genes to achieve production of benzoate via a β-oxidation pathway. Strategic disruption of the native Pseudomonas benzoate degradation pathway further allowed the production of catechol and cis,cis-muconate. Taken together, this work demonstrates new routes for the microbial production of these industrially relevant chemicals from renewable resources.
14 May 2020Submitted to Biotechnology Journal
18 May 2020Submission Checks Completed
18 May 2020Assigned to Editor
19 May 2020Reviewer(s) Assigned
16 Jun 2020Editorial Decision: Revise Minor
25 Jun 20201st Revision Received
28 Jun 2020Submission Checks Completed
28 Jun 2020Assigned to Editor
28 Jun 2020Reviewer(s) Assigned
21 Jul 2020Editorial Decision: Accept
Nov 2020Published in Biotechnology Journal volume 15 issue 11 on pages 2000211. 10.1002/biot.202000211